Journal: International Journal of Molecular Sciences

Article Title: NLRP3-Mediated Piezo1 Upregulation in ACC Inhibitory Parvalbumin-Expressing Interneurons Is Involved in Pain Processing after Peripheral Nerve Injury

doi: 10.3390/ijms232113035

Figure Lengend Snippet: SNI activates the morphological transformation of microglia into amoeba-like cells. ( A ) Representative immunofluorescence staining image of Iba1-IR (microglial marker, magenta) in the bilateral ACC of sham and SNI groups. Scale bar = 50 μm. ( B ) Comparison of the process lengths and cell body areas of microglia in the bilateral ACC of sham and SNI groups. * p -value < 0.05, ** p -value < 0.01, *** p -value < 0.001 versus the sham group (unpaired t test). ( C ) Left: representative immunofluorescence staining image showing CD68-IR in the bilateral ACC in both sham and SNI rats. Scale bar = 50 μm. Right: quantification of the CD68 fluorescence signals in both groups. ** p -value < 0.01 versus the sham group (two-way ANOVA). ( D ) White arrow heads indicate the co-localization of CD68-IR (red) with that of vGAT (green, top) and PV (green, below) 7d post SNI by double staining. Scale bar = 25 μm in the top line; 50 μm in the bottom line. Blue fluorescence in (A, C and D) is from DAPI, a nuclear counterstain.

Article Snippet: GraphPad Software was used for multiple t -test or unpaired t -test or one-way ANOVA or two-way ANOVA to determine the differences over different groups where appropriate.

Techniques: Transformation Assay, Immunofluorescence, Staining, Marker, Comparison, Fluorescence, Double Staining